Composite

Part:BBa_K4146507:Design

Designed by: Yujie Zhang   Group: iGEM22_BUCT   (2022-09-16)


pCS-aroGfbr


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 450
    Illegal EcoRI site found at 665
    Illegal XbaI site found at 87
    Illegal XbaI site found at 941
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 450
    Illegal EcoRI site found at 665
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 450
    Illegal EcoRI site found at 665
    Illegal BamHI site found at 738
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 450
    Illegal EcoRI site found at 665
    Illegal XbaI site found at 87
    Illegal XbaI site found at 941
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 450
    Illegal EcoRI site found at 665
    Illegal XbaI site found at 87
    Illegal XbaI site found at 941
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

site-specific mutagenesis, insertional mutagenesis


Source

Escherichia coli

References